LOCUS       ISEc29-mph(E)-IS26_unit 6647 bp    DNA     linear       19-OCT-2020
DEFINITION  Citrobacter freundii.
ACCESSION
VERSION
KEYWORDS    .
SOURCE      Citrobacter freundii
  ORGANISM  Citrobacter freundii
            Unclassified.
REFERENCE   1  (bases 1 to 6647)
  AUTHORS   Zhou,D.
  TITLE     Complete sequence of ISEc29-mph(E)-IS26_unit
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6647)
  AUTHORS   Zhou,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-OCT-2020) State Key Laboratory of Pathogen and
            Biosecurity, Beijing Institute of Microbiology and Epidemiology,
            Beijing, Beijing 100071, China
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: newbler v. 2.6
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6647
                     /organism="Citrobacter freundii"
                     /mol_type="genomic DNA"
     mobile_element  1..6647
                     /gene="ISEc29-mph(E)-IS26 unit"
                     /product="Putative resistance unit: ISEc29-mph(E)-IS26
                     unit"
     mobile_element  1..1325
                     /gene="ISEc29"
                     /product="Insertion sequence: ISEc29"
     repeat_region   1..21
                     /gene="IRL_ISEc29"
                     /product="ISEc29 inverted repeat left"
     CDS             107..1291
                     /gene="tnpA"
                     /codon_start=1
                     /transl_table=11
                     /product="ISEc29 transposase"
                     /translation="MLTMNVKAMLADFLTFVTPKSMHKARFSVLLDAVTALAKDACCT
                     VTAIGRAMPGSSDKVSIKRADRLLNNPNLQRELPLIYAALTASIVGHKTKPMILVDWS
                     NADTAKRHFILRASIAADGRALTLLQKIAAAEDYTCPHLHGAFLKQLKAMLPKDCKPV
                     IVTDAGFKVPWLKQVRKLGWHYVARVRGNVKLKLAEQDKFISVNQLYRQAKKDPKSVG
                     KIMLAQTQHYETQAVLVGKGYKLLKRDKNKTYKEPWLLVSSLADCHGYADKIAKCYSS
                     RMQIEESFRDQKSHRYGLGSDLHGTKKKSRLEILLLLAALVNWFHYLLGSAAEKAGLH
                     LRYQANTVKNRRVLALNFLGILLCKEPKQRIRRQYYQQGLKQILQWVVQWDWAVIKQA
                     DS"
     repeat_region   1305..1325
                     /gene="IRR_ISEc29"
                     /product="ISEc29 inverted repeat right"
     CDS             1690..3165
                     /gene="msr(E)"
                     /codon_start=1
                     /transl_table=11
                     /product="Macrolide efflux protein"
                     /translation="MSLIIKARNIRLDYAGRDVLDIDELEIHSYDRIGLVGDNGAGKS
                     SLLKVLNGEIVLAEATLQRFGDFAHISQLGGIEIETVEDRAMLSRLGVSNVQNDTMSG
                     GEETRAKIAAAFSQQVHGILADEPTSHLDLNGIDLLIGQLKAFDGALLVISHDRYFLD
                     MVVDKIWELKDGKITEYWGGYSDYLRQKEEERQHQAVEYELMMKERERLESAVQEKRQ
                     QANRLDNKKKGEKSKNSTESAGRLGHAKMTGTKQRKLYQAAKSMEKRLAALEDIQAPE
                     HLRSIRFRQSSALELHNKFPITADGLSLKFGSRTIFDDANFIIPLGAKVAITGSNGTG
                     KTSLLKMISERADGLTISPKAEIGYFTQTGYKFNTHKSVLSFMQEECEYTVAEIRAVL
                     ASMGIGANDIQKNLSDLSGGEIIKLLLSKMLLGKYNILLMDEPGNYLDLKSIAALETM
                     MKSYAGTIIFVSHDKQLVDNIADIIYEIKDHKIIKTFERDC"
     misc_feature    3221..4105
                     /gene="mph(E)"
                     /product="Macrolide 2'-phosphotransferase"
     CDS             complement(4464..5006)
                     /gene="orf543"
                     /codon_start=1
                     /transl_table=11
                     /product="Hypothetical protein"
                     /translation="MKKLSVSELAKLYGYSRQAIYAHINKGNLSKGSDGLIDFSEALR
                     VFGEPQKKEDTVNQSQSINSQNLTEVDLLKRQVDILEKQLNQAIQRENQSLERESFYQ
                     EQIEAMQRLLEAPKANMTTFTDQSFKQDIATDPRSELATNYDELTTPQQDNKRIPIPE
                     HVEPEQKKRGFLSRFFLPYG"
     CDS             complement(5073..5900)
                     /gene="repAciN"
                     /codon_start=1
                     /transl_table=11
                     /product="Plasmid replication protein RepA"
                     /translation="MGSCAAPSAKGDDKFITTDYLQQCRVHRNTAYQALKDACDDLFA
                     RQFSYQSLSEKGNTINHKSRWVSEVAYIDNEAVVRLIFAPAIVPLITRLEEQFTKYEI
                     QQISNLTSAYAVRLYEILIAWRSTGKTPLITMYDFRQKIGVLETEYKRMYDFKKYVLD
                     IALKQVNEHTDIIVKVEQHKTGRSITGFSFSFKQKKSATHSVESKRDPNTLDLFSKIT
                     DKQRHLFANKLSELPEMSKYSQGTESYQQFAVRIAAMLQDAEKFKELLPLLRKLGFQ"
     repeat_region   5828..5841
                     /gene="IRL_IS26"
                     /product="IS26 inverted repeat left"
     CDS             5891..6595
                     /gene="tnpA"
                     /codon_start=1
                     /transl_table=11
                     /product="IS26 transposase"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDH
                     STIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTV
                     DFYLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDV
                     EHRQIKYRNNVIECDHGKLKRIINATLGFKSMKTAYATIKGIEVMRALRKGQASAFYY
                     GDPLGEMRLVSRVFEM"
     repeat_region   6634..6647
                     /gene="IRR_IS26"
                     /product="IS26 inverted repeat right"
BASE COUNT     1987 a   1254 c   1429 g   1977 t
ORIGIN
        1 ctgaggtatc cccagaaatt ctgtagttaa aataaaatgt tagttgattg gggaacattc
       61 atggcggttc ctgatctgat cattcgccaa aactcccaca ccgcggatgc tcaccatgaa
      121 tgtaaaagct atgctcgctg atttcctcac ctttgtcacg ccaaaatcaa tgcataaagc
      181 ccgattttcg gttttgctag atgcggtaac cgccctggca aaggatgcgt gttgcaccgt
      241 tactgcgatt ggccgggcga tgcctggctc ttcagataag gtcagtatca aacgggcaga
      301 tcgtctactc aataatccta acctgcaacg agagctgcca ttgatttatg ctgcgctgac
      361 ggcttctatt gttggccata aaactaagcc gatgattttg gttgactgga gcaatgccga
      421 tactgccaag cgacacttta tcctgcgtgc cagcattgcc gctgacggta gagcgttgac
      481 tctgctacag aagattgccg ccgcagaaga ttatacctgc ccacacctac acggggcgtt
      541 tttaaagcag cttaaagcca tgctacccaa ggactgtaag cccgtaattg tcactgacgc
      601 ggggtttaaa gttccatggc tgaaacaagt gcgtaagctg ggatggcatt atgtagctag
      661 agttcgaggc aatgtgaagc ttaaattggc agagcaggac aagtttatca gtgttaatca
      721 gctttatcgg caagcgaaga aggatccaaa aagtgtagga aaaatcatgc ttgcccaaac
      781 acaacactat gaaacgcagg ccgtcctggt tggcaaaggt tataagctat tgaaacgcga
      841 taaaaataag acgtataagg aaccatggtt gttggtgtca tccttagctg actgccatgg
      901 gtatgcggat aaaattgcta agtgctacag tagccgaatg caaattgaag aaagcttccg
      961 tgatcagaaa agtcaccgct atggcctggg tagcgatttg catggtacca agaagaaatc
     1021 tcgcttagag atactgctac tactggccgc attggttaat tggtttcatt acctgctagg
     1081 tagcgcagcg gagaaagcgg gtttgcacct gcgttatcaa gctaacaccg ttaaaaatag
     1141 gcgggtattg gccctgaatt tccttgggat attactttgc aaagaaccca aacagcgaat
     1201 acgcaggcaa tattatcagc agggacttaa acaaatacta cagtgggtgg ttcagtggga
     1261 ctgggcagta atcaaacagg ctgatagctg attgtatgaa tggaaatttt gtggggatcc
     1321 ctcaggttgt ctagacttct tttaacagta aagttatcat aaaactgaat tttatttttt
     1381 aggtaagttt atgcattcta tccgcattcg ttaagacaca actatttgca tagtgacact
     1441 attttataat ggtgggcttt tgttgtgtct ttaagaatat atgcggatat ataaagtaaa
     1501 agtatgctta atttataagt atgcttttag tgcatagttt ccagttataa cttaattgac
     1561 tagctatttg tccaccctgt ggatgaatag cttttttttt gggaggacac tgtgatgcta
     1621 gcttttgttt tcacctaaat cctgtttgct gcataaaaaa tttcaagagc taaacaggag
     1681 taaataaaaa tgagtttaat tattaaagcg agaaacatac gcttggatta tgctgggcgt
     1741 gatgttttgg atattgatga attggaaatt cactcttatg accgtattgg tcttgtgggt
     1801 gataacggag caggaaagag tagtttactc aaagtactta atggcgaaat tgttttagcc
     1861 gaagcgacat tacagcgttt tggtgatttt gcacatatca gccaactggg cggaatcgaa
     1921 atagaaacgg tcgaagaccg ggcaatgtta tctcgccttg gtgtttccaa tgtacaaaac
     1981 gacacaatga gtggcggaga ggaaactcgt gcaaaaattg ctgccgcatt ttcccaacaa
     2041 gtacatggca ttctagcgga tgaaccaacc agccaccttg atctcaatgg aatagatcta
     2101 cttattggtc aacttaaagc atttgatgga gcattacttg ttatcagtca tgaccgatat
     2161 tttcttgata tggttgtaga caagatatgg gagttaaaag acggtaaaat tacggaatat
     2221 tggggtggtt actcggatta cttgcgtcaa aaagaagaag agcgacaaca ccaagccgta
     2281 gaatatgagc tgatgatgaa ggaacgggag cgattagaat ctgctgtgca agaaaaacgc
     2341 cagcaagcta atcgattaga caataagaaa aaaggagaaa aatccaaaaa ctctaccgaa
     2401 agtgctggac gacttgggca tgcaaaaatg actggcacca agcaaagaaa actgtatcag
     2461 gcagctaaga gtatggaaaa gcgtttggct gcattagaag atattcaagc accagagcat
     2521 ttgcgttcta ttcgttttcg tcaaagttca gccctagaac tgcacaataa gttcccgatt
     2581 acggcagatg gtctgagctt aaaatttggt agccgtacta tctttgatga cgctaacttt
     2641 ataataccgc ttggcgctaa agtcgctata actggatcga atggaacagg gaaaacgtcc
     2701 ttgttaaaaa tgatatcaga acgtgctgat ggattaacca tatctccaaa agctgaaatt
     2761 ggctacttta cacaaacagg atataaattt aacacgcata aatctgtgct ctcctttatg
     2821 caggaagagt gcgagtacac agttgcggaa attcgtgcag tattggcttc aatggggatc
     2881 ggagcgaatg atattcaaaa aaacttatcc gacttatcgg gaggtgaaat catcaaactg
     2941 cttttatcca aaatgctttt aggaaaatat aatattttgc ttatggatga accaggaaac
     3001 tatcttgacc taaaaagtat tgccgcatta gaaacaatga tgaagtccta tgcaggaact
     3061 attatcttcg tatctcatga caagcaattg gtcgataata ttgctgacat tatctacgag
     3121 atcaaagacc acaaaatcat caagactttt gagagagatt gttaatgata gccaatctaa
     3181 tccgaacatt aattattgaa ctctttaaag gaaattaaaa atgacaattc aagatattca
     3241 atcacttgct gaagcacacg gcttgttgct tacggacaaa atgaatttca atgaaatggg
     3301 cattgatttt aaggtcgttt ttgctcttga tacaaagggg caacaatggt tgctgcgtat
     3361 tcctcgtcgt gatggcatga gggaacaaat caagaaagaa aaacgcattt tagaattggt
     3421 aaaaaaacat ctttctgtag aggttcctga ttggtgaatt tcatctacag aattagtggc
     3481 ttatcccata cttaaagata atcctgtttt aaatttggat gctgaaacct atgaaataat
     3541 ttggaatatg gacaaagata gcccgaaata cataacatct ttggcaaaaa ccttatttga
     3601 aatccatagt attcctgaaa aagaagttcg ggaaaatgat ttgaaaatta tgaaaccttc
     3661 agatttaaga cctgaaatag caaacaattt gcagttagta aaatctgaaa ttggtataag
     3721 tgagcaattg gaaacccgct acagaaaatg gttggataat gatgttctat gggcagattt
     3781 cacccaattt atacatggcg atttatatgc tgggcatgta ctagcttcaa aggatggagc
     3841 tgtttcaggc gttattgatt ggtcaacagc ccatatagat gacccagcga ttgattttgc
     3901 tgggcatgta actttgtttg gagaagaaag cctcaaaact ctaatcatcg agtatgaaaa
     3961 actagggggt aaagtttgga ataaactata tgaacagact ttagaaagag cagcggcctc
     4021 tcctttgatg tatggtttat ttgccttaga aactcaaaat gaaagcctta tcgttggagc
     4081 aaaagctcag ttgggagtta tataatttaa aaatatgatt gctgagaact gccttgtttt
     4141 gaaacttggt tggctttaat tagtttttag tattctttat agaaaatgcc tcgatcaagg
     4201 ggcatttcta acaatcattt aacataaaat ttcttatacg aaatgcttga tatttctctt
     4261 taaatatcat tatattaacg taagccgttc tggagtatag ggcaccagaa cggcttttta
     4321 ttgattattc atgttccacg atcactaatt gagcgatgtt ccacggtttt attcagtttt
     4381 aataaacatc atccggtttt ttgaggagtc attatttcaa gtgcttgcac tgaaattggc
     4441 tcttcaaaaa actggagtct tggctaacca tatggaagga aaaagcggct taggaagcct
     4501 ctcttcttct gttctggttc aacatgctcc gggataggaa tacgcttatt gtcttgctga
     4561 ggagtagtca attcgtcata gttcgttgct agttccgacc gaggatccgt tgctatatcc
     4621 tgtttaaagc tctgatcggt aaaggtagtc atattggctt ttggtgcttc tagtaagcgc
     4681 tgcatagctt caatctgttc ttgataaaac gattcacgtt ctaaagattg attttctctc
     4741 tgtattgcct gatttaattg tttttccagt atgtcaactt gacgttttag taagtcaact
     4801 tctgtcaagt tttgactgtt aattgattga ctttgattga ctgtatcctc ttttttttgt
     4861 ggttccccaa aaactcttag ggcctctgaa aagtcaatta atccatcaga tcctttagat
     4921 aaatttcctt tgtttatatg tgcatatatg gcttgtcttg aatatccata aagcttagct
     4981 aactctgaaa ctgacagttt tttcattatg taaaccagtt ttcaacttgt gttaatacct
     5041 gactgttaac ttaacaattt caattaacaa tgtcattgaa atcctaattt tctgagtaaa
     5101 ggaagtaatt ccttaaattt ctctgcatct tgcagcatgg cagctatacg tacagcaaac
     5161 tgctgatagc tttctgtgcc ttgtgaatat ttactcatct caggaagctc tgagagcttg
     5221 ttggcgaata gatggcgttg tttatctgtt atttttgaaa agaggtctaa tgtattcgga
     5281 tctcttttag attcgactga atgcgtggct gattttttct gtttaaagct aaatgaaaaa
     5341 ccagtaatag atctacctgt tttatgctgt tcaactttga caataatatc ggtatgttca
     5401 tttacttgtt ttaatgcaat gtctaaaaca tattttttaa aatcatacat tcgtttgtat
     5461 tcagtctcga gtacacctat tttttgtcta aaatcataca tagttatgag aggcgttttt
     5521 ccagtactac gccatgcaat caatatttca tataaacgaa cagcataagc acttgttaaa
     5581 ttacttattt gttgtatttc atactttgta aattgttctt ctaacctagt aattaaaggc
     5641 acaatagcag gggcaaaaat aagtctaacg acagcttcat tatcaatata agccacctcg
     5701 ctcacccatc ttgatttgtg attaatagtg ttaccttttt cactaagact ctgataactg
     5761 aattgtcttg caaacaagtc atcgcaagca tcttttaacg cctgataagc cgtatttcga
     5821 tgtacacggc actgttgcaa atagtcggtg gtgataaact tatcatcccc ttttgctgat
     5881 ggagctgcac atgaacccat tcaaaggccg gcattttcag cgtgacatca ttctgtgggc
     5941 cgtacgctgg tactgcaaat acggcatcag ttaccgtgag ctgcaggaga tgctggctga
     6001 acgcggagtg aatgtcgatc actccacgat ttaccgctgg gttcagcgtt atgcgcctga
     6061 aatggaaaaa cggctgcgct ggtactggcg taacccttcc gatctttgcc cgtggcacat
     6121 ggatgaaacc tacgtgaagg tcaatggccg ctgggcgtat ctgtaccggg ccgtcgacag
     6181 ccggggccgc actgtcgatt tttatctctc ctcccgtcgt aacagcaaag ctgcataccg
     6241 gtttctgggt aaaatcctca acaacgtgaa gaagtggcag atcccgcgat tcatcaacac
     6301 ggataaagcg cccgcctatg gtcgcgcgct tgctctgctc aaacgcgaag gccggtgccc
     6361 gtctgacgtt gaacaccgac agattaagta ccggaacaac gtgattgaat gcgatcatgg
     6421 caaactgaaa cggataatca acgccacgct gggatttaaa tccatgaaga cggcttacgc
     6481 caccatcaaa ggtattgagg tgatgcgtgc actacgcaaa ggccaggcct cagcatttta
     6541 ttatggtgat cccctgggcg aaatgcgcct ggtaagcaga gtttttgaaa tgtaaggcct
     6601 ttgaataaga caaaaggctg cctcatcgct aactttgcaa cagtgcc
//
